Difference between revisions of "Part:BBa K1045010:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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For cloning of the inverse RBS [[Part:BBa_K1045010|BBa_K1045010]], hybridization oligos for the RBS [[Part:BBa_B0034|BBa_B0034]] were used. Prefix and Suffix were switched to yield an inverted RBS.
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For cloning of the inverse RBS [[Part:BBa_K1045010|BBa_K1045010]], hybridization oligos for the RBS [[Part:BBa_B0034|BBa_B0034]] were used. In these hybridization oligos, Prefix and Suffix were switched to yield an inverted RBS.
  
 
===Source===
 
===Source===

Revision as of 19:36, 4 October 2013


RBS BBa_B0034 with inversed Pre- and Suffix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For cloning of the inverse RBS BBa_K1045010, hybridization oligos for the RBS BBa_B0034 were used. In these hybridization oligos, Prefix and Suffix were switched to yield an inverted RBS.

Source

The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_B0034.

References