Difference between revisions of "Part:BBa K1045002:Design"

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===Design Notes===
 
===Design Notes===
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone (see [[Part:BBa_K1045004|BBa_K1045004]]). It was then further ''SpeI'' and ''EcoRI'' digested and ligated with the ''XbaI'' and ''EcoRI'' digested CFP (BBa-E0020). Altogether this composes [[Part:BBa_K1045002|BBa_K1045002]].
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone (see [[Part:BBa_K1045004|BBa_K1045004]]). It was then further ''SpeI'' and ''EcoRI'' digested and ligated with the ''XbaI'' and ''EcoRI'' digested CFP ([[Part:BBa_E0020|BBa_E0020]]). Altogether this composes [[Part:BBa_K1045002|BBa_K1045002]].
  
 
===Source===
 
===Source===

Revision as of 19:31, 4 October 2013

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone (see BBa_K1045004). It was then further SpeI and EcoRI digested and ligated with the XbaI and EcoRI digested CFP (BBa_E0020). Altogether this composes BBa_K1045002.

Source

This composite part consists of parts either derived from PCR amplification of B. subtilis chromosomal DNA or from the distribution kit 2013. For more information, see "Design Notes" above.

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965