Difference between revisions of "Part:BBa K1123000"
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This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project. Within our project we were focusing on the expression of MRI contrast providing proteins under anaerobic conditions. As we wished these anaerobic conditions to be the trigger for the protein expression we needed a promoter which would react to this environment and ultimately induce the protein expression. The FNR promoter is a naturally occurring promoter which exists in almost all strains of E.coli which makes it an extremely attractive system to use.  
 
This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project. Within our project we were focusing on the expression of MRI contrast providing proteins under anaerobic conditions. As we wished these anaerobic conditions to be the trigger for the protein expression we needed a promoter which would react to this environment and ultimately induce the protein expression. The FNR promoter is a naturally occurring promoter which exists in almost all strains of E.coli which makes it an extremely attractive system to use.  
  
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Upon entering a hypoxic environment the forming of [4Fe-4S]2+ dimers will be stimulated within FNR producing bacteria. These dimers are the transcription activators of the FNR promoter. They are able to bind to the promoter allowing the promoter to become active. Important to note is that the promoter we are using is a tandem promoter meaning that 2 [4Fe-4S]2+ dimers are able to bind to the promoter. This tandem effect enhances the induction effect of the promoter, ultimately leading to a higher expression rate.
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As it is not possible to quantify the workings of a promoter without expression
  
  

Revision as of 19:26, 4 October 2013

FNR Promoter

This part is a standard FNR promoter. It has two FNR binding sites at -41.5 and at -91.5 with 0 being the transcriptional starting point of this promoter. The promoter can be used in E.coli strains to induce protein expression in anaerobic environments. It is natively used to induce metabolic processes when the E.coli bacteria enter anaerobic environments. The part can be used to express any protein sequence placed behind it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project. Within our project we were focusing on the expression of MRI contrast providing proteins under anaerobic conditions. As we wished these anaerobic conditions to be the trigger for the protein expression we needed a promoter which would react to this environment and ultimately induce the protein expression. The FNR promoter is a naturally occurring promoter which exists in almost all strains of E.coli which makes it an extremely attractive system to use.

Upon entering a hypoxic environment the forming of [4Fe-4S]2+ dimers will be stimulated within FNR producing bacteria. These dimers are the transcription activators of the FNR promoter. They are able to bind to the promoter allowing the promoter to become active. Important to note is that the promoter we are using is a tandem promoter meaning that 2 [4Fe-4S]2+ dimers are able to bind to the promoter. This tandem effect enhances the induction effect of the promoter, ultimately leading to a higher expression rate.

As it is not possible to quantify the workings of a promoter without expression