Difference between revisions of "Part:BBa K1045012:Experience"
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===Applications of BBa_K1045012=== | ===Applications of BBa_K1045012=== | ||
| − | This composite part seems to be funtional, as the characterization of our DarR reporter system [[Part:BBa_K1045017|BBa_K1045017]] indicated. We saw that from the control plasmid [[Part:BBa_K1045013|BBa_K1045013]] harboring this part upstream of GFP coding sequence (derived from [[Part:BBa_E0240|BBa_E0240]]) GFP is expressed. This shows that the promoter is active in absence of DarR [[Part:BBa_K1045001|BBa_K1045001]]. Moreover, expression of DarR shuts down ''gfp'' transcription indicating that the operator [[Part:BBa_K1045000|BBa_K1045000]]is functional. | + | This composite part seems to be funtional, as the characterization of our DarR reporter system [[Part:BBa_K1045017|BBa_K1045017]] indicated. We saw that from the control plasmid [[Part:BBa_K1045013|BBa_K1045013]] harboring this part upstream of GFP coding sequence (derived from [[Part:BBa_E0240|BBa_E0240]]) GFP is expressed. This shows that the promoter is active in absence of DarR [[Part:BBa_K1045001|BBa_K1045001]]. Moreover, expression of DarR shuts down ''gfp'' transcription indicating that the operator [[Part:BBa_K1045000|BBa_K1045000]]is functional. For more information see: [https://parts.igem.org/Part:BBa_K1045017:Experience]. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:06, 4 October 2013
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Applications of BBa_K1045012
This composite part seems to be funtional, as the characterization of our DarR reporter system BBa_K1045017 indicated. We saw that from the control plasmid BBa_K1045013 harboring this part upstream of GFP coding sequence (derived from BBa_E0240) GFP is expressed. This shows that the promoter is active in absence of DarR BBa_K1045001. Moreover, expression of DarR shuts down gfp transcription indicating that the operator BBa_K1045000is functional. For more information see: [1].
User Reviews
UNIQ383bcda29b445161-partinfo-00000000-QINU UNIQ383bcda29b445161-partinfo-00000001-QINU