Difference between revisions of "Part:BBa K1033120"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1033120 short</partinfo>
 
<partinfo>BBa_K1033120 short</partinfo>
  
−
Construct needed to produce the protein miraculin, but fitted with a, export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
+
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This has historically been used as a sweetener.
 +
 
 +
This part is a sunthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
 +
 
 +
==Results==
 +
We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:02, 4 October 2013

B0034-ExpTag-Linker-Miraculin-Linker-6xHis

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This has historically been used as a sweetener.

This part is a sunthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Results

We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]