Difference between revisions of "Part:BBa K1123000"
Line 6: Line 6:
 
The part can be used to express any protein sequence placed behind it.  
 
The part can be used to express any protein sequence placed behind it.  
  
−
<!-- Add more about the biology of this part here -->
+
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
Line 14: Line 14:
  
 
===Characterization===
 
===Characterization===
−
This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project.  
+
This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project. Within our project we were focusing on the expression of MRI contrast providing proteins under anaerobic conditions. As we wished these anaerobic conditions to be the trigger for the protein expression we needed a promoter which would react to this environment and ultimately induce the protein expression. The FNR promoter is a naturally occurring promoter which exists in almost all strains of E.coli which makes it an extremely attractive system to use.
 +
 
 +
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 18:58, 4 October 2013

FNR Promoter

This part is a standard FNR promoter. It has two FNR binding sites at -41.5 and at -91.5 with 0 being the transcriptional starting point of this promoter. The promoter can be used in E.coli strains to induce protein expression in anaerobic environments. It is natively used to induce metabolic processes when the E.coli bacteria enter anaerobic environments. The part can be used to express any protein sequence placed behind it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project. Within our project we were focusing on the expression of MRI contrast providing proteins under anaerobic conditions. As we wished these anaerobic conditions to be the trigger for the protein expression we needed a promoter which would react to this environment and ultimately induce the protein expression. The FNR promoter is a naturally occurring promoter which exists in almost all strains of E.coli which makes it an extremely attractive system to use.