Difference between revisions of "Part:BBa K1045011:Design"
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===Source=== | ===Source=== | ||
| − | This part originated from hybridization oligos purchased from Sigma-Aldrich. The promoter sequence is that of [[Part:BBa_J23117|BBa_J23117]] given on its Parts Registry page, while the 54 additional bases represent a random sequence that would translate into the amino acid sequence cyclicdiampacterim. | + | This part originated from hybridization oligos purchased from Sigma-Aldrich. The promoter sequence is that of [[Part:BBa_J23117|BBa_J23117]] given on its Parts Registry page, while the 54 additional bases represent a random sequence that would translate into the amino acid sequence "cyclicdiampacterim". |
===References=== | ===References=== | ||
Latest revision as of 18:51, 4 October 2013
Promoter reverse
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
Illegal NheI site found at 24 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 55
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To construct this part, hybridization oligos were used. These hybridization oligos encoded the sequence of BBa_J23117 and 54 additional bases upstream of this promoter. The insert generated by the hybridization of the oligos was cloned into pSB1C3 via EcoRI and SpeI.
Source
This part originated from hybridization oligos purchased from Sigma-Aldrich. The promoter sequence is that of BBa_J23117 given on its Parts Registry page, while the 54 additional bases represent a random sequence that would translate into the amino acid sequence "cyclicdiampacterim".