Difference between revisions of "Part:BBa K1152000:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
This is just the coding sequence. We sequenced with VR and VF2 primer, no mutations were found (http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip).
 
This is just the coding sequence. We sequenced with VR and VF2 primer, no mutations were found (http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip).
−
You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new peptide.
+
You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new non-ribosomal peptide.
 
Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts
 
Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts
 
[[File:Heidelberg_RFC10_TycASeq.zip]]
 
[[File:Heidelberg_RFC10_TycASeq.zip]]

Latest revision as of 18:23, 4 October 2013

TycA_deltaCom Module from Tyrocidine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518


Design Notes

This is just the coding sequence. We sequenced with VR and VF2 primer, no mutations were found (http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip). You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new non-ribosomal peptide. Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts File:Heidelberg RFC10 TycASeq.zip

Source

Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835

References