Difference between revisions of "Part:BBa K1152001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | This is just the coding sequence. | ||
Silent mutation was found after sequencing with VR and VF2 at positions 4595 (GCC to GCG; Alanin to Alanin) and at position 4598 (CTT to CTG; Leucine to Leucine)(http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip and http://2013.igem.org/Team:Heidelberg/Tyrocidine_week20_biobrickmod#Sequencing ). | Silent mutation was found after sequencing with VR and VF2 at positions 4595 (GCC to GCG; Alanin to Alanin) and at position 4598 (CTT to CTG; Leucine to Leucine)(http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip and http://2013.igem.org/Team:Heidelberg/Tyrocidine_week20_biobrickmod#Sequencing ). | ||
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| + | You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new peptide. | ||
| + | Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts | ||
===Source=== | ===Source=== | ||
Latest revision as of 18:22, 4 October 2013
TycB1 Module from Tyrocidine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1195
Design Notes
This is just the coding sequence. Silent mutation was found after sequencing with VR and VF2 at positions 4595 (GCC to GCG; Alanin to Alanin) and at position 4598 (CTT to CTG; Leucine to Leucine)(http://2013.igem.org/File:Heidelberg_RFC10_TycASeq.zip and http://2013.igem.org/Team:Heidelberg/Tyrocidine_week20_biobrickmod#Sequencing ).
You can use it to combine it with other modules of e.g. the Tyrocidine cluster for the construction of a new peptide.
Go to our wiki: http://2013.igem.org/Team:Heidelberg/Parts
Source
Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835