Difference between revisions of "Part:BBa K1028002:Design"
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The parts were assembled using PCR assembly to avoid introducing a scar site between the promoter and the ribosome binding site included in part BBa_K081012 and in assembled parallel | The parts were assembled using PCR assembly to avoid introducing a scar site between the promoter and the ribosome binding site included in part BBa_K081012 and in assembled parallel | ||
| + | This part uses a strong RBS to maximize fluorescence response following activation of pCpxR. | ||
Latest revision as of 18:07, 4 October 2013
A cell membrane stress reporter gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 728
Design Notes
The parts were assembled using PCR assembly to avoid introducing a scar site between the promoter and the ribosome binding site included in part BBa_K081012 and in assembled parallel
This part uses a strong RBS to maximize fluorescence response following activation of pCpxR.
Source
The parts for this were aquired from the iGEM 2013 delivery plates.