Difference between revisions of "Part:BBa K1028003:Design"
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<partinfo>BBa_K1028003 short</partinfo> | <partinfo>BBa_K1028003 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
The biobricks were digested and ligated together using the RFC10 restriction sites | The biobricks were digested and ligated together using the RFC10 restriction sites | ||
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| + | This part uses a strong RBS to both maximize production of the INP.Si4 complex and the fluorescence response of GFP production from activation of pCpxR | ||
Latest revision as of 18:06, 4 October 2013
Silica Detection System
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 62
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 472
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1917
Design Notes
The biobricks were digested and ligated together using the RFC10 restriction sites
This part uses a strong RBS to both maximize production of the INP.Si4 complex and the fluorescence response of GFP production from activation of pCpxR
Source
The DNA for this part was taken from the registry