Difference between revisions of "Part:BBa K1150021"
Line 27: Line 27:
 
|}
 
|}
  
−
[[File:Freiburg2013 Plasmid Cas9-KRAB-1.jpg|800px|thumb|center|<b>Fig. 1</b> Schematic overview of dCas9-KRAB composite part with all features.]]  
+
[[File:Freiburg2013 Plasmid Cas9-KRAB-1.jpg|800px|thumb|left|<b>Fig. 1</b> Schematic overview of dCas9-KRAB composite part with all features.]]  
  
  
−
<!-- Add more about the biology of this part here
+
==Usage and Biology==
−
===Usage and Biology===
+
  
−
==Sequence and Features==
 
−
<partinfo>BBa_K1150021 SequenceAndFeatures</partinfo>
 
  
 +
<span class='h3bb'>==Sequence and Features==</span>
 +
<partinfo>BBa_K1150021 SequenceAndFeatures</partinfo>
  
−
<!-- Uncomment this to enable Functional Parameter display
+
==Functional Parameters==
−
===Functional Parameters===
+
 
<partinfo>BBa_K1150021 parameters</partinfo>
 
<partinfo>BBa_K1150021 parameters</partinfo>
−
<!-- -->
 

Revision as of 17:45, 4 October 2013

uniCAS Repressor (SV40 promoter)

SV40:HA-NLS-dCas9-Linker-KRAB-NLS:BGH
Function Transcriptional Repression
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes, Homo sapiens
Source Feng Zhang, Addgene
Konrad Müller, University of Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]
Fig. 1 Schematic overview of dCas9-KRAB composite part with all features.


Usage and Biology

==Sequence and Features==


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4784
    Illegal SapI.rc site found at 4748

Functional Parameters