Difference between revisions of "Part:BBa K1123001"
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<partinfo>BBa_K1123001 short</partinfo> | <partinfo>BBa_K1123001 short</partinfo> | ||
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<partinfo>BBa_K1123001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1123001 SequenceAndFeatures</partinfo> | ||
| + | ===Characterization=== | ||
| + | This part is a composite part which is comprised of a number of other important parts namely [[Part: BBa_K1123000 | BBa_K1123000]] and [[Part: BBa_K1123013 | BBa_K1123013]] | ||
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Revision as of 17:42, 4 October 2013
Protamine-1-Optimized Protein Production Construct
This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Protamine-1-Optimized Protein sequence. The FNR promotor sequence enables us to bring the Protamine-1-Optimized protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The Protamine-1-Optimized protein is a human protein which we are expressing with the sole purpose of using it as a CEST contrast agent.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 200
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 39
Illegal XhoI site found at 359 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This part is a composite part which is comprised of a number of other important parts namely BBa_K1123000 and BBa_K1123013