Difference between revisions of "Part:BBa K1033103"
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== Geranyl diphosphate syntease (IspA) == | == Geranyl diphosphate syntease (IspA) == | ||
Revision as of 17:38, 4 October 2013
_NOTOC_ geranyl diphosphate synthase
Geranyl diphosphate syntease (IspA)
Catalyses the formation of dimethylallyl diphophate + isopentenyl diphosphate to geranyl diphosphate. As this is an often rate-limiting step in production of isoprenoids, it can desirable to introduce additional copies of the gene to increase yield.
This part was cloned from the chromosome of a d5alpha e.coli with overhangs containing ribosome binding site B0034 and biobrick restriction site.
Results
We were unsuccessful in assembling any promoter to the construct, and as such could not further characterize the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 886
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 182
- 1000COMPATIBLE WITH RFC[1000]