Difference between revisions of "Part:BBa K1060009"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1060009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1060009 SequenceAndFeatures</partinfo>
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==Construct digestion==
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https://static.igem.org/mediawiki/2013/a/a1/BBa_K1060009_gel_pic.jpg
 
https://static.igem.org/mediawiki/2013/a/a1/BBa_K1060009_gel_pic.jpg
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http://www.youtube.com/watch?v=n-pMp8-XdUo
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==System testing==
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[http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]
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For more infromation see experience or ADD LINK.

Revision as of 17:37, 4 October 2013

Medium constitutive expression of EBF synthase from Artemisia annua

For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 177
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal BamHI site found at 677
    Illegal XhoI site found at 639
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 249


Construct digestion

BBa_K1060009_gel_pic.jpg


System testing

[http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]

For more infromation see experience or ADD LINK.