Difference between revisions of "Part:BBa K1150021"
Line 2: Line 2:
 
<partinfo>BBa_K1150021 short</partinfo>
 
<partinfo>BBa_K1150021 short</partinfo>
  
−
{| style="color:black" cellpadding="7" cellspacing="1" border="1" align="right"
+
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
−
! colspan="2" style="background:#FFBF00;"|KRAB
+
! colspan="2" style="background:#FFBF00;"|SV40:HA-NLS-dCas9-Linker-KRAB-NLS:BGH
 
|-
 
|-
 
|'''Function'''
 
|'''Function'''
−
|Repressor domain
+
|Transcriptional Repression
 
|-
 
|-
 
|'''Use in'''
 
|'''Use in'''
 
|Mammalian cells
 
|Mammalian cells
−
|-
 
−
|'''Organism'''
 
−
|<i>Homo sapiens</i>
 
 
|-
 
|-
 
|'''RFC standard'''
 
|'''RFC standard'''
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|-
 
|-
 
|'''Backbone'''
 
|'''Backbone'''
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|pSB1C3
+
|pSB1C3<br>
 +
|-
 +
|'''Organism'''
 +
|<i>Streptococcus pyogenes, Mus musculus</i>
 
|-
 
|-
 
|'''Source'''
 
|'''Source'''
−
|Konrad Müller
+
|Feng Zhang, Addgene<br> Konrad, University of Freiburg
 
|-
 
|-
 
|'''Submitted by'''
 
|'''Submitted by'''

Revision as of 17:20, 4 October 2013

uniCAS Repressor (SV40 promoter)

SV40:HA-NLS-dCas9-Linker-KRAB-NLS:BGH
Function Transcriptional Repression
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes, Mus musculus
Source Feng Zhang, Addgene
Konrad, University of Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4784
    Illegal SapI.rc site found at 4748