Difference between revisions of "Part:BBa K1141000"

 
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<partinfo>BBa_K1141000 short</partinfo>
 
<partinfo>BBa_K1141000 short</partinfo>
  
 
This part contains all the necessary DNA to produce the red fluorescent protein mCherry with the PLac promoter. PLac repression is low in most E. coli strains and the promoter will be leaky. Efficient repression can be implemented by additionally transforming a strain with the pREP4 plasmid from the M15 E. coli strain of QIAGEN, or transforming the part into this strain.
 
This part contains all the necessary DNA to produce the red fluorescent protein mCherry with the PLac promoter. PLac repression is low in most E. coli strains and the promoter will be leaky. Efficient repression can be implemented by additionally transforming a strain with the pREP4 plasmid from the M15 E. coli strain of QIAGEN, or transforming the part into this strain.
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The figure below shows the excitation and emission spectra as can be obtained from CHROMA's spectra viewer:
  
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<p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6c/Spectra_mCherry.png" alt="Excitation-Emission spectra for mCherry"></p>
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<p align="center" id="legend">Figure 1: Excitation (left peak) and Emission (right peak) spectra for mCherry.</p>
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===Usage and Biology===
 
===Usage and Biology===
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The part can be used as a fluorescent reporter (see excitation and emission spectra for details on which filters to use). mCherry is part of the mFruits. Its chromophore has a fast maturation speed (~30 minutes according to our observations), and it is resistant to photobleaching as shown by experiments during which it was illuminated with a <a href="http://www.topled.fr/Eclairage_domestique/Ampoule_MR16/index.html">6W 12V LED lamp</a> (search for AMPOULE 6 Watts - COB HAUTE PUISSANCE - 520 LUMENS - MR16 (GU5.3) - 12v - BLANC NATUREL, the site is French).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 17:12, 4 October 2013

Plac-RBS-mCherry-double terminator (IPTG-inducible)

This part contains all the necessary DNA to produce the red fluorescent protein mCherry with the PLac promoter. PLac repression is low in most E. coli strains and the promoter will be leaky. Efficient repression can be implemented by additionally transforming a strain with the pREP4 plasmid from the M15 E. coli strain of QIAGEN, or transforming the part into this strain. The figure below shows the excitation and emission spectra as can be obtained from CHROMA's spectra viewer:

Excitation-Emission spectra for mCherry

Figure 1: Excitation (left peak) and Emission (right peak) spectra for mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]