Difference between revisions of "Part:BBa K1093000"

 
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<partinfo>BBa_K1093000 short</partinfo>
 
<partinfo>BBa_K1093000 short</partinfo>
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Emission peak: 440 nm
 
Emission peak: 440 nm
  
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Team Warsaw 2013 measured sfBFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.
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[[File:Superfolderwykres.jpg|center|594px|border|caption]]
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''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
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{| class="wikitable"
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|-
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! scope="col"| -
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! colspan="3" | Samples
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! scope="col" | Arithmetic mean
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! scope="col" | Standard deviation
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|-
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|sfGFP
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|100,60
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|100,37
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|99,02
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|100,00
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|0,85
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|-
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|sfCFP
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|17,95
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|18,38
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|17,36
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|17,90
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|0,51
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|-
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|sfBFP
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|40,65
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|41,52
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|42,25
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|41,47
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|0,80
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|-
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|sfYFP
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|218,87
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|216,51
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|217,87
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|217,75
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|1,19
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|-
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|}
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''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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sfBFP can be used as reporter.
  
 
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Revision as of 17:07, 4 October 2013

sfBFP

Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm

Team Warsaw 2013 measured sfBFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.

caption

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

- Samples Arithmetic mean Standard deviation
sfGFP 100,60 100,37 99,02 100,00 0,85
sfCFP 17,95 18,38 17,36 17,90 0,51
sfBFP 40,65 41,52 42,25 41,47 0,80
sfYFP 218,87 216,51 217,87 217,75 1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13