Difference between revisions of "Part:BBa K1123019"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1123019 short</partinfo>
 
<partinfo>BBa_K1123019 short</partinfo>
Line 12: Line 11:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1123019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1123019 SequenceAndFeatures</partinfo>
 +
 +
===Characterization===
 +
This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.
  
  

Revision as of 16:38, 4 October 2013

Poly(Arginine-Serine) Protein

This part contains the DNA sequence of a protein of our own design. We first repeated the Arginine-Serine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added. The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI. The protein can be placed behind a promoter of your choice and expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part was created for use in the 2013 TU-Eindhoven iGEM project. Unfortunately however we did not get round to completing a proper characterization for this part as it went largely unused.