Difference between revisions of "Part:BBa K1141001"

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This biobrick contains the PLac and RBS sequences from QIAGEN's pQE30 plasmid in front of the red fluorescent protein KillerRed. KillerRed has an additional function as a photosensitizer and the protein can be used to kill cells if illuminated by strong light while expressing KillerRed. KillerRed's presence in a culture can be seen (red colour similar to RFP), and the level of fluorescence corresponds to the amount of protein. Fluorescence peaks are 535 nm for absorption and 610 nm for emission. The protein dimerizes with itself and is not suitable for protein fusions.
 
This biobrick contains the PLac and RBS sequences from QIAGEN's pQE30 plasmid in front of the red fluorescent protein KillerRed. KillerRed has an additional function as a photosensitizer and the protein can be used to kill cells if illuminated by strong light while expressing KillerRed. KillerRed's presence in a culture can be seen (red colour similar to RFP), and the level of fluorescence corresponds to the amount of protein. Fluorescence peaks are 535 nm for absorption and 610 nm for emission. The protein dimerizes with itself and is not suitable for protein fusions.
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All characterization data is included on the page for <a href="https://parts.igem.org/Part:BBa_K1141002">BBa_K1141002</a> which codes for the same protein, but which is in the correct biobrick plasmid pSB1C3 and which has the Eco RI site removed to make the sequence RFC[10]-compatible.
 
All characterization data is included on the page for <a href="https://parts.igem.org/Part:BBa_K1141002">BBa_K1141002</a> which codes for the same protein, but which is in the correct biobrick plasmid pSB1C3 and which has the Eco RI site removed to make the sequence RFC[10]-compatible.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 16:07, 4 October 2013

Plac-RBS-KillerRed (IPTG-inducible, pQE30 plasmid format)

This biobrick contains the PLac and RBS sequences from QIAGEN's pQE30 plasmid in front of the red fluorescent protein KillerRed. KillerRed has an additional function as a photosensitizer and the protein can be used to kill cells if illuminated by strong light while expressing KillerRed. KillerRed's presence in a culture can be seen (red colour similar to RFP), and the level of fluorescence corresponds to the amount of protein. Fluorescence peaks are 535 nm for absorption and 610 nm for emission. The protein dimerizes with itself and is not suitable for protein fusions. All characterization data is included on the page for BBa_K1141002 which codes for the same protein, but which is in the correct biobrick plasmid pSB1C3 and which has the Eco RI site removed to make the sequence RFC[10]-compatible. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 82
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
    Illegal BamHI site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 295
    Illegal BsaI.rc site found at 586