Difference between revisions of "Part:BBa K1022127:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1022127 short</partinfo>
 
<partinfo>BBa_K1022127 short</partinfo>
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===Design Notes===
 
===Design Notes===
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'''Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft] '''
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Latest revision as of 15:54, 4 October 2013

pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 2317
    Illegal NheI site found at 4624
    Illegal NheI site found at 6719
    Illegal NheI site found at 6742
    Illegal NotI site found at 5320
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BglII site found at 2441
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1405
    Illegal BamHI site found at 5289
    Illegal XhoI site found at 5298
    Illegal XhoI site found at 5329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2626
    Illegal AgeI site found at 6324
    Illegal AgeI site found at 6394
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 6975


Design Notes

Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft]

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Source

Clonned

References