Difference between revisions of "Part:BBa K1022121:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1022121 short</partinfo>
 
<partinfo>BBa_K1022121 short</partinfo>
Line 8: Line 7:
 
===Design Notes===
 
===Design Notes===
 
The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119
 
The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119
 +
 +
'''Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft] '''
 +
 +
  
  

Latest revision as of 15:47, 4 October 2013

pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 2317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BglII site found at 2441
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1405
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2626
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119

Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft]



Source

Clonned

References