Difference between revisions of "Part:BBa K1141002"

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Note that the information characterising this part was made with BBa_K1141001, which has the same sequence except for an additional Eco RI site between the Promoter and RBS. The vector for BBa_K1141001 is a high copy plasmid like pSB1C3 with ac col1 as duplication origin.
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Note that the information characterising this part was made with BBa_K1141001, which has the same sequence except for an additional Eco RI site between the Promoter and RBS. The vector for BBa_K1141001 is a high copy plasmid like pSB1C3 with ac col1 as duplication origin. KillerRed made by this biobrick dimerizes and isn't suitable for protein fusions.
  
 
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Revision as of 15:45, 4 October 2013

Plac-RBS-KillerRed (IPTG-inducible)

KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI).With this biobrick the production of KillerRed is IPTG-inducible. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013. KillerRed is a red fluorescent protein with Absorption in the green portion and emission in the red portion of the visible spectrum (figure 1):

Killer Red absorption-emission spectra

Figure 1:The KillerRed protein absorption (left peak) and emission (right peak) spectra
Source: Detailed KillerRed description from Evrogen

Note that the information characterising this part was made with BBa_K1141001, which has the same sequence except for an additional Eco RI site between the Promoter and RBS. The vector for BBa_K1141001 is a high copy plasmid like pSB1C3 with ac col1 as duplication origin. KillerRed made by this biobrick dimerizes and isn't suitable for protein fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 289
    Illegal BsaI.rc site found at 580