Difference between revisions of "Part:BBa K1141002"
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KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI).With this biobrick the production of KillerRed is IPTG-inducible. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013. | KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI).With this biobrick the production of KillerRed is IPTG-inducible. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013. | ||
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| − | KillerRed is a red fluorescent protein with Absorption in the green portion and emission in the red portion of the visible spectrum:<br><br> | + | KillerRed is a red fluorescent protein with Absorption in the green portion and emission in the red portion of the visible spectrum (figure 1):<br><br> |
<p align="center"><img src="https://static.igem.org/mediawiki/2013/7/7f/KillerRed_spectra2.png" alt="Killer Red absorption-emission spectra" width="500px"></p> | <p align="center"><img src="https://static.igem.org/mediawiki/2013/7/7f/KillerRed_spectra2.png" alt="Killer Red absorption-emission spectra" width="500px"></p> | ||
| − | <p align="center" id="legend">The KillerRed protein absorption (left peak) and emission (right peak) spectra<br> | + | <p align="center" id="legend">Figure 1:The KillerRed protein absorption (left peak) and emission (right peak) spectra<br> |
Source: <a href="http://www.evrogen.com/products/KillerRed/KillerRed_Detailed_description.shtml">Detailed KillerRed description from Evrogen</a><br><br></p> | Source: <a href="http://www.evrogen.com/products/KillerRed/KillerRed_Detailed_description.shtml">Detailed KillerRed description from Evrogen</a><br><br></p> | ||
Revision as of 15:37, 4 October 2013
Plac-RBS-KillerRed (IPTG-inducible)
KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI).With this biobrick the production of KillerRed is IPTG-inducible. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013.
KillerRed is a red fluorescent protein with Absorption in the green portion and emission in the red portion of the visible spectrum (figure 1):
Figure 1:The KillerRed protein absorption (left peak) and emission (right peak) spectra
Source: Detailed KillerRed description from Evrogen
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 133
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 289
Illegal BsaI.rc site found at 580