Difference between revisions of "Part:BBa K1111006:Design"
 
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<partinfo>BBa_K1111006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1111006 SequenceAndFeatures</partinfo>
  
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The primers used for the amplification of the MP2 CDS also added a His-tag to the 5' end and a linker to the 3' end of the CDS.
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The primers used for the amplification of the MMP2 CDS also added a His-tag to the 5' end and a linker to the 3' end of the CDS.
  
 
Link for the MMP2 plasmid:http://plasmid.med.harvard.edu/PLASMID/GetCloneDetail.do?cloneid=2849&species=Homo%20sapiens <BR>
 
Link for the MMP2 plasmid:http://plasmid.med.harvard.edu/PLASMID/GetCloneDetail.do?cloneid=2849&species=Homo%20sapiens <BR>

Latest revision as of 15:06, 4 October 2013

Matrix metalloprotease 2 (MMP2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The primers used for the amplification of the MMP2 CDS also added a His-tag to the 5' end and a linker to the 3' end of the CDS.

Link for the MMP2 plasmid:http://plasmid.med.harvard.edu/PLASMID/GetCloneDetail.do?cloneid=2849&species=Homo%20sapiens
MMP2 forward primer:5'-ATG CACCACCACCACCACCAC GAG GCG CTA ATG GCC C-3'
MMP2 reverse primer:5'-ACC ACC AGA TTG AAA ATA CAA ATT TTC ACC GCA GCC TAG CCA GTC GGA TTT GAT-3'

PCR Result:

Figure 2: PCR: MMP2 insert




























Design Notes

In order to activate the enzyme a special buffer is needed. We also added a his tag to the protein to purify it.


Source

Homo sapiens

References