Difference between revisions of "Part:BBa K1141002"

Revision as of 14:43, 4 October 2013 (view source) Simon.pacouret (Talk | contribs) (Added absorption/emission spectra) ← Older edit		Revision as of 14:44, 4 October 2013 (view source) Simon.pacouret (Talk | contribs) Newer edit →
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	<p align="center"><img src="https://static.igem.org/mediawiki/2013/7/7f/KillerRed_spectra2.png" alt="Killer Red absorption-emission spectra" width="500px"></p>		<p align="center"><img src="https://static.igem.org/mediawiki/2013/7/7f/KillerRed_spectra2.png" alt="Killer Red absorption-emission spectra" width="500px"></p>
−	<p id="legend">Figure 1.<br>The KillerRed protein absorption (left peak) and emission (right peak) spectra<br>	+	<p align="center" id="legend">The KillerRed protein absorption (left peak) and emission (right peak) spectra<br>
	Source: <a href="http://www.evrogen.com/products/KillerRed/KillerRed_Detailed_description.shtml">Detailed KillerRed description from Evrogen</a><br><br></p>		Source: <a href="http://www.evrogen.com/products/KillerRed/KillerRed_Detailed_description.shtml">Detailed KillerRed description from Evrogen</a><br><br></p>

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Revision as of 14:44, 4 October 2013

Plac-RBS-KillerRed (IPTG-inducible)

KillerRed is a red fluorescent protein which produces ROS under green illumination (roughly 500-600 nm, absorption peak at 535 nm). This biobrick allows production of KillerRed under the control of the PLAC promoter (In this construction, actually a T5 phage promoter preceded by two lac operators for strong repression in presence of LacI).With this biobrick the production of KillerRed is IPTG-inducible. We used this part to trigger cell death in response to light illumination for controlling cell density in the Talk'E.coli project of Grenoble-EMSE-LSU 2013. KillerRed is a red fluorescent protein with Absorption in the green portion and emission in the red portion of the visible spectrum:

The KillerRed protein absorption (left peak) and emission (right peak) spectra
Source: Detailed KillerRed description from Evrogen

Note that the information characterising this part was made with BBa_K1141001, which has the same sequence except for an additional Eco RI site between the Promoter and RBS. The vector for BBa_K1141001 is a high copy plasmid like pSB1C3 with ac col1 as duplication origin.

Sequence and Features