Difference between revisions of "Part:BBa K1111003:Design"

 
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===Design Notes===
 
===Design Notes===
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I took 500bp upstream from the beginning of the target gene
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The Promoter was isolated directly from the Genimic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon.
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+
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===Source===
 
===Source===

Revision as of 13:48, 4 October 2013


Cad Promoter induced by low pH and high lysine conentrations


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 474


Design Notes

The Promoter was isolated directly from the Genimic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon.

Source

E.coli, Substrain MG1655

References