Difference between revisions of "Part:BBa K1111005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Because the promoter is very short (35bp) we designed it's primers with overhangs that were complementary to the pBS1C3 plasmid. This allowed us to insert the promoter into the plasmid by Gibson Assembly. | |
Revision as of 13:37, 4 October 2013
Constitutive promoter inducing GFP expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 74
Design Notes
Because the promoter is very short (35bp) we designed it's primers with overhangs that were complementary to the pBS1C3 plasmid. This allowed us to insert the promoter into the plasmid by Gibson Assembly.
Source
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