Difference between revisions of "Part:BBa K1150006"

Revision as of 13:23, 4 October 2013

UVR8

Sunlight is essential for plants: Not only as an energy source but also as information for their growth cycle. Therefore they need receptors to detect different light wavelengths. Ultraviolet B (UVB) light can be perceived with photoreceptor UV resistance Locus 8 (UVR8) [1]. This receptor derives drom Arapidopsis thaliana and contains its core domain (amino acids 12-381) [2]. The receptor reacts best to light of the wavelenght 311 nm.

Biology and Usage

In the absence of UVB light UVR8 forms homodimers. This is its closed configuration. When UVB light is perceived UVR8 monomerizes and changes into its open configuration. UVR8 is then able to recruit COP1 [2]. This interaction is disrupted when UVB radiation stops. Plants use this mechanism to perceive UVB light and respond with photomorphogenesis or react to UVB induced stress [3].

Freiburg 2013 used this part to engineer a UVB light induced mechanism to activate gene expression. You need a combination of these BioBricks: Cas9-UVR8, COP1-VP16, RNaimer

Sequence and Features

References

[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332

[2] Müller K., Engesser R., Schulz S., Steinberg T., Tomakidi P., Weber C. C., Ulm R., Timmer J., Zurbriggen M. D., Weber W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Research Vol. 41, No. 12

[3] Favory J.-J., Stec A., Gruber H., Rizzini L., Oravecz A., Funk M., Albert A., Cloix C., Jenkins G. I., Oakeley E. J., Seidlitz H. K., Nagy F., Ulm R. (2009). Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. The EMBO Journal 28, 591-600