Difference between revisions of "Part:BBa K1150037"
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|RNAimer (VEGF4 target)
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! colspan="2" style="background:#FFBF00;"|RNAimer (VEGF2 target)
 
|-
 
|-
 
|'''Function'''
 
|'''Function'''
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|Targeting VEGF4 with Cas9
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|Targeting VEGF2 with Cas9
 
|-
 
|-
 
|'''Use in'''
 
|'''Use in'''
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This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF4 target (GTGTGCAGACGGCAGTCACTAGGGGGCGCT).
+
This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF2 target (GTGTGCAGACGGCAGTCACTAGGGGGCGCT).
  
 
==Biology and Usage==
 
==Biology and Usage==
  
−
Freiburg 2013 used this plasmid to test the efficiency of target VEGF4. Therefore this target was also cloned in front of a promoter, that on its part is located in front of a SEAP reporter gene.
+
Freiburg 2013 used this plasmid to test the efficiency of target VEGF2. Therefore this target was also cloned in front of a promoter, that on its part is located in front of a SEAP reporter gene.
  
 
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Revision as of 13:10, 4 October 2013

uniCAS RNaimer to target VEGF2


RNAimer (VEGF2 target)
Function Targeting VEGF2 with Cas9
Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF2 target (GTGTGCAGACGGCAGTCACTAGGGGGCGCT).

Biology and Usage

Freiburg 2013 used this plasmid to test the efficiency of target VEGF2. Therefore this target was also cloned in front of a promoter, that on its part is located in front of a SEAP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216

Literature