Difference between revisions of "Part:BBa K1104100"
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===Usage and Biology===
 
===Usage and Biology===
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The mannose degradation pathway is often seen in many organisms, and the reason why we choose from MG1655 is that this species of ''E-coli'' are able to live in bees' midgut. Besides MG1655 is also the bacteria that we are going to transform our whole circuit into.  This decision make our experiment more easier and more certain that this enzyme can well perform in our ''Bee. coli''.  We tent to overexpress this gene in order to inhibit the production of the PTP1 in the polar filament of ''N. ceranae'', the chosen target fungi that we are eager to eliminate.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:52, 4 October 2013

mngB - alpha-mannosidase

mngB, a passage of gene in Escherichia coli K-12 substr MG1655, can produce alpha-mannosidase. It is an enzyme that turns 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate, which is the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 716
    Illegal BglII site found at 992
    Illegal BglII site found at 2139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
    Illegal AgeI site found at 2242
  • 1000
    COMPATIBLE WITH RFC[1000]