Difference between revisions of "Part:BBa K1075045"
 
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<partinfo>BBa_K1075045 short</partinfo>
 
<partinfo>BBa_K1075045 short</partinfo>
  
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This part is a combination of <partinfo>BBa_K1075044</partinfo> and the fluorescent protein mCherry[http://www.nature.com/nbt/journal/v22/n12/full/nbt1037.html].  
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This part is a combination of the light-inducible <partinfo>BBa_K1075044</partinfo> and the fluorescent protein mCherry[http://www.nature.com/nbt/journal/v22/n12/full/nbt1037.html].  
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 09:26, 4 October 2013

pDawn-RBS34-mCherry-TT

This part is a combination of the light-inducible BBa_K1075044 and the fluorescent protein mCherry[http://www.nature.com/nbt/journal/v22/n12/full/nbt1037.html].

Usage and Biology

pDawn-RBS34-mCherry-TT can be used to photocontrol the expression of the fluorescent protein mCherry. In this system pDawn (BBa_K1075044) can be regulated by blue light, while mCherry acts as reporter protein. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3048
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 940
    Illegal NgoMIV site found at 1072
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1953
    Illegal NgoMIV site found at 1971
    Illegal NgoMIV site found at 2061
    Illegal AgeI site found at 1291
    Illegal AgeI site found at 2419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2520
    Illegal BsaI.rc site found at 1402