Difference between revisions of "Part:BBa K1075045:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part is a combination of <partinfo>BBa_K1075045</partinfo> and the fluorescent protein mCherry. | + | This part is a combination of the light-inducible <partinfo>BBa_K1075045</partinfo> and the fluorescent protein mCherry. |
mCherry is a fluorescent protein with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. It matures quickly, with a t<sub>0.5</sub> of 15 minutes, allowing it to be visualised soon after translation. (Shaner et al. 2004) | mCherry is a fluorescent protein with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. It matures quickly, with a t<sub>0.5</sub> of 15 minutes, allowing it to be visualised soon after translation. (Shaner et al. 2004) | ||
Latest revision as of 09:25, 4 October 2013
pDawn-RBS34-mCherry-TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3048
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 940
Illegal NgoMIV site found at 1072
Illegal NgoMIV site found at 1166
Illegal NgoMIV site found at 1459
Illegal NgoMIV site found at 1953
Illegal NgoMIV site found at 1971
Illegal NgoMIV site found at 2061
Illegal AgeI site found at 1291
Illegal AgeI site found at 2419 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2520
Illegal BsaI.rc site found at 1402
Design Notes
This part is a combination of the light-inducible BBa_K1075045 and the fluorescent protein mCherry.
mCherry is a fluorescent protein with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. It matures quickly, with a t0.5 of 15 minutes, allowing it to be visualised soon after translation. (Shaner et al. 2004)
Source
mCherry is derived from a protein isolated from Discosoma sp..
References
[http://www.nature.com/nbt/journal/v22/n12/full/nbt1037.html]Shaner, Nathan C; Campbell, Robert E; Steinbach, Paul A; Giepmans, Ben N G; Palmer, Amy E; Tsien, Roger Y (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein.