Difference between revisions of "Part:BBa K1088003"
Line 4: Line 4:
 
This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)
 
This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)
  
−
References
+
Reference: see design source
−
 
+
−
(1) Molecular cloning, expression and characterization of cDNA
+
−
encoding cis-prenyltransferases from Hevea brasiliensis :  
+
−
Kasem Asawatreratanakul, Seiji Takahashi, Yuan-Wei Zhang, Atiya Rattanapittayaporn, Dhirayos Wititsuwannakul, Rapepun Wititsuwannakul, and Tanetoshi Koyama
+
  
  

Revision as of 00:03, 4 October 2013

Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA

This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)

Reference: see design source


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]