Difference between revisions of "Part:BBa K1088007"
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The purpose of the part was to test the expression profile of ''dxs'' from the lac promter. BBa_K1088012 is a similar part that does not contain the linker-GFP part.  
 
The purpose of the part was to test the expression profile of ''dxs'' from the lac promter. BBa_K1088012 is a similar part that does not contain the linker-GFP part.  
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Fluorescence activated cell sorting (FACS) was used to examine the expression profile the a similar reporter fusion (''dxs'' drerived from ''B. subtilis'': BBa_K1088008). The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation).
 
  
 
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Revision as of 23:59, 3 October 2013

E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)

This part consist of the dxs gene derived from E. coli fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS.

The purpose of the part was to test the expression profile of dxs from the lac promter. BBa_K1088012 is a similar part that does not contain the linker-GFP part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2756
    Illegal SapI.rc site found at 946