Difference between revisions of "Part:BBa K1065303:Design"
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===Source=== | ===Source=== | ||
| − | YF1 is a fusion protein of YtvA (from B subtilis) and FixL (from B japonicum); FixJ and pFixK2 come from the genome of B. japonicum. | + | YF1 is a fusion protein of YtvA (from <i>B subtilis </i>) and FixL (from <i>B japonicum</i>); FixJ and pFixK2 come from the genome of <i>B. japonicum<7i>. |
The part consists of:<BR> | The part consists of:<BR> | ||
<partinfo>BBa_K592016 </partinfo><BR> | <partinfo>BBa_K592016 </partinfo><BR> | ||
Revision as of 20:41, 3 October 2013
B0034-YF1-B0034-FixJ-pFixK2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 634
Illegal NgoMIV site found at 724
Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 1254
Illegal NgoMIV site found at 1547
Illegal NgoMIV site found at 1641
Illegal AgeI site found at 276
Illegal AgeI site found at 1422 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1311
Illegal BsaI.rc site found at 175
Design Notes
This part has been made following the standard assembly, by inserting BBa_K592006 inside BBa_K592016
Source
YF1 is a fusion protein of YtvA (from B subtilis ) and FixL (from B japonicum); FixJ and pFixK2 come from the genome of B. japonicum<7i>.
The part consists of:
BBa_K592016
BBa_K592006
References
1) Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
2)Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542