Difference between revisions of "Part:BBa K1127000"

Revision as of 20:29, 3 October 2013

A3-Flg Gold binding peptide

A de novo synthesized gene with A3 and Flg domains which should hypothetically bind to gold and form nanoparticles.

Characterization

For peptide activity testing , we cloned the peptides under the constitutive promoter (BBa_J23100). Characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any size down to 10nm. The technology is based on optical properties as well as Brownian motion of the particles. Please visit http://www.nanosight.com/ for more information.

We obtained a series of recordings and several analytical reports of the particles within samples from the overnight cultures of E. coli strain DH5 alpha transformed with the hybrids. Unfortunately, the results can't provide evidence for gold bio-mineralization because changes were subtle and not significant compared to the controls (untransformed E. coli). Please visit out Protocols section for more detail [http://2013.igem.org/Team:York_UK/Notebook.html?page=protocols].

File:PelB-A3-Flg- STOP.mp4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 78
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ==Characterization==
-We created a set of hybrid parts (BBa_J23100 + BBa_K1127000-3) for characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any  size down to 10nm.  The technology is based on optical properties as well as Brownian motion of the particles.  Please visit http://www.nanosight.com/ for more information.
+For peptide activity testing , we cloned the peptides under the constitutive promoter (BBa_J23100). Characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any  size down to 10nm.  The technology is based on optical properties as well as Brownian motion of the particles.  Please visit http://www.nanosight.com/ for more information.
 We obtained a series of recordings and several analytical reports of the particles within samples from the overnight cultures of E. coli strain DH5 alpha transformed with the hybrids. Unfortunately, the results can't provide evidence for gold bio-mineralization because changes were subtle and not significant compared to the controls (untransformed E. coli). Please visit out Protocols section for more detail [http://2013.igem.org/Team:York_UK/Notebook.html?page=protocols].
 [[File:PelB-A3-Flg- STOP.mp4|center]]