Difference between revisions of "Part:BBa K1127002"

Revision as of 20:25, 3 October 2013

IM9 and A3-Flg fusion for gold binding

This is the inverted version of the part BBa_K1127020 with the same function.

Characterization

We created a set of hybrid parts (BBa_J23100 + BBa_K1127000-3) for characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any size down to 10nm. The technology is based on optical properties as well as Brownian motion of the particles. Please visit http://www.nanosight.com/ for more information.

We obtained a series of recordings and several analytical reports of the particles within samples from the overnight cultures of E. coli strain DH5 alpha transformed with the hybrids. Unfortunately, the results can't provide evidence for gold bio-mineralization because changes were subtle and not significant compared to the controls (untransformed E. coli). Please visit out Protocols section for more detail [http://2013.igem.org/Team:York_UK/Notebook.html?page=protocols].

File:PelB-IM9-Link-A3-Flg- STOP.mp4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 78
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ==Characterization==
-We tried to characterize the part using Nanoparticle Tracking Analyzer from NanoSight [http://www.nanosight.com]. The part was cloned into BBa_J23100 [https://parts.igem.org/Part:BBa_J23100] which allows constitutive expression of the peptide.
+We created a set of hybrid parts (BBa_J23100 + BBa_K1127000-3) for characterization using Nanoparticle Tracking Analyzer (NanoSight LM10 ®). The device allows us to visualize, measure and characterize any type of nanoparticles and any  size down to 10nm.  The technology is based on optical properties as well as Brownian motion of the particles.  Please visit http://www.nanosight.com/ for more information.
+We obtained a series of recordings and several analytical reports of the particles within samples from the overnight cultures of E. coli strain DH5 alpha transformed with the hybrids. Unfortunately, the results can't provide evidence for gold bio-mineralization because changes were subtle and not significant compared to the controls (untransformed E. coli). Please visit out Protocols section for more detail [http://2013.igem.org/Team:York_UK/Notebook.html?page=protocols].
-We obtained recording (below) and several analytical reports of the particles within samples from the overnight cultures (E. coli strain DH5 alpha + BBa_K1127002/BBa_J23100 hybrid). The results can't provide evidence for gold bio-mineralization by the peptide because changes were subtle and not significant compared to the controls. Please visit our wiki page for more information [http://2013.igem.org/Team:York_UK].
 [[File:PelB-IM9-Link-A3-Flg-_STOP.mp4]]