Difference between revisions of "Part:BBa J100122"
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<partinfo>BBa_J100122 short</partinfo> | <partinfo>BBa_J100122 short</partinfo> | ||
| − | This is a strong promoter driving transcription of theophylline riboswitch D followed by E. coli optimized adhE gene. Used in combination with adhE deletion strain SHH31. | + | This is a strong promoter driving transcription of theophylline riboswitch D followed by E. coli optimized adhE gene. Used in combination with adhE deletion strain SHH31. Deletion of a Valine codon between bp 176 and 177. |
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Latest revision as of 20:23, 3 October 2013
P5+riboswitch+adhE
This is a strong promoter driving transcription of theophylline riboswitch D followed by E. coli optimized adhE gene. Used in combination with adhE deletion strain SHH31. Deletion of a Valine codon between bp 176 and 177.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 649
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 101
Illegal AgeI site found at 734
Illegal AgeI site found at 1958
Illegal AgeI site found at 2003 - 1000COMPATIBLE WITH RFC[1000]