Difference between revisions of "Part:BBa K1075028"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo> pJD427-plac2-RBS34-mcherry-TT short</partinfo>
+
<partinfo>BBa_K1075028 short</partinfo>
  
  

Revision as of 19:34, 3 October 2013

SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-TT


construction

The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a ecSspB split system.


biology

The plasmid pJD427 contains the two parts of splitted ecSspB, which can be fused (and thus regain function) by addition of rapamycin. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/


plac is a IPTG inducible promoter.


The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.


mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum at 610 nm. http://www.ncbi.nlm.nih.gov/pubmed/15558047


The double terminator stops the transcription at this point.


application

This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ecssrA(DAS+4)-TT plasmid. [[1]]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 102
    Illegal BsaI.rc site found at 743