Difference between revisions of "Part:BBa K1075021"

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<partinfo>BBa_K1075021 short</partinfo>
 
<partinfo>BBa_K1075021 short</partinfo>
  
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ecssrA(DAS+4)-TT
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E. coli ssrA(DAS+4)-TT
  
 
===construction===
 
===construction===
−
The part contains the mutated ecssrA (DAS+4) tag and a double terminator.
+
The part contains the mutated E. coli(ec)ssrA (DAS+4) tag and a double terminator.
  
 
===biology===
 
===biology===
−
The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ssrA (DAS+4) weakens a direct binding between proteases and ssrA and increases the dependance of sspB.[http://www.ncbi.nlm.nih.gov/pubmed/16762842]
+
The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA (DAS+4) and increases the dependance of sspB.[http://www.ncbi.nlm.nih.gov/pubmed/16762842]
  
 
The double terminator stops the transcription at this point.
 
The double terminator stops the transcription at this point.
  
 
===application===
 
===application===
−
The ssrA tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.
+
The ecssrA(DAS+4) tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.
  
  

Revision as of 18:51, 3 October 2013

E. coli ssrA(DAS+4)-TT

E. coli ssrA(DAS+4)-TT

construction

The part contains the mutated E. coli(ec)ssrA (DAS+4) tag and a double terminator.

biology

The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA (DAS+4) and increases the dependance of sspB.[http://www.ncbi.nlm.nih.gov/pubmed/16762842]

The double terminator stops the transcription at this point.

application

The ecssrA(DAS+4) tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]