Difference between revisions of "Part:BBa K1075020"
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===biology=== | ===biology=== | ||
| − | The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated | + | The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA(DAS+4) and increases the dependance of sspB. [http://www.ncbi.nlm.nih.gov/pubmed/16762842] |
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===application=== | ===application=== | ||
Revision as of 18:41, 3 October 2013
E. coli ssrA(DAS+4)
construction
The part contains the mutated E. coli ssrA(DAS+4) tag.
biology
The ssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor proteins SspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA(DAS+4) and increases the dependance of sspB. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]
application
The ssrA tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 762
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]