Difference between revisions of "Part:BBa K1216007"

Revision as of 18:17, 3 October 2013

Variant of the wild-type pLuxR promoter with lower sensitivity

BBa_K1216007 is a variant of the wild-type pLuxR promoter with a lowered sensitivity for LuxR-AHL.

Comparison of BBa_R0062 to the mutant

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Site directed mutagenesis of the BBa_R0062 pLuxR wild type promoter=

Our goal was to achieve mutations in the luxbox of the pLuxR BBa_R0062 promoter to shift the dose response curve of the promoter depending on the OHHL (3-oxo-hexanoyl-l-homoserine-l-lactone) concentration.
We did site directed mutagenisis of specific sites of the luxbox according to the results of the literature ()

Figure 1. Site of the directed mutagenesis in the luxbox.As indicated you can see the mutations of the pLuxR variant compared to the wild type in red. The positions we mutated randomly are indicated in red-underlined

@@ Line 18: / Line 18: @@
 <!-- -->
-===Characterization of the pLuxR===
+=== Site directed mutagenesis of the BBa_R0062 pLuxR wild type promoter====
+Our goal was to achieve  mutations in the luxbox of the pLuxR BBa_R0062 promoter to shift the dose response curve of the promoter depending on the OHHL (3-oxo-hexanoyl-l-homoserine-l-lactone) concentration.
+<br> We did site directed mutagenisis of specific sites of the luxbox according to the results of the literature ()
+[[File:promoterscheme.jpg|400px|left|thumb|<b>Figure 1. Site of the directed mutagenesis in the luxbox.</b>As indicated you can see the mutations of the pLuxR variant compared to the wild type in red. The positions we mutated randomly are indicated in red-underlined]]
+===Characterization of the pLuxR variant dose response to OHHL using plate reader analysis and FACS single cell analysis===