Difference between revisions of "Part:BBa K1223013:Experience"
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===Applications of BBa_K1223013=== | ===Applications of BBa_K1223013=== | ||
| + | This part was used by us to incorporate the unnatural amino acid propargyl-L-lysine into various proteins. | ||
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| + | in the fluorescent gel we show incorporation of the unnatural amino acid into copper oxidase of E.coli in various loactions | ||
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| + | H117,N262,D411,M412 - position and amino acid that was replaced with the UAA. | ||
| + | CueO - native protein without UAA incorporated. | ||
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| + | [[File:UAA.jpg]] | ||
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| + | we used a reactive fluorescent dye to identify the incorporated UAA in the gel. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:06, 3 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1223013
This part was used by us to incorporate the unnatural amino acid propargyl-L-lysine into various proteins.
in the fluorescent gel we show incorporation of the unnatural amino acid into copper oxidase of E.coli in various loactions
H117,N262,D411,M412 - position and amino acid that was replaced with the UAA. CueO - native protein without UAA incorporated.
we used a reactive fluorescent dye to identify the incorporated UAA in the gel.
User Reviews
UNIQ84b6c39e72b6f8d3-partinfo-00000000-QINU UNIQ84b6c39e72b6f8d3-partinfo-00000001-QINU