Difference between revisions of "Part:BBa K1075028"

Revision as of 16:05, 3 October 2013

No part name specified with partinfo tag.

construction

The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system.

biology

The plasmid pJD427 contains the two parts of splitted SspB, which can be fused (and thus regain function) by addition of rapamycin. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/

plac is a IPTG inducible promoter.

The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.

mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum at 610 nm. http://www.ncbi.nlm.nih.gov/pubmed/15558047

The double terminator stops the transcription at this point.

application

This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ssrA-TT plasmid. [[1]]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1174
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 102
Illegal BsaI.rc site found at 743

@@ Line 10: / Line 10: @@
 ===biology===
 The plasmid pJD427 contains the two parts of splitted SspB, which can be fused (and thus regain function) by addition of rapamycin. [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/]]
 plac is a IPTG inducible promoter.
 The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.
 mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum  at 610 nm.
 [[http://www.ncbi.nlm.nih.gov/pubmed/15558047]]
 The double terminator stops the transcription at this point.
 ===application===