Difference between revisions of "Part:BBa K1075028"
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | <partinfo> | + | <partinfo> pJD427-plac2-RBS34-mcherry-TT short</partinfo> |
| − | |||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===construction=== | ===construction=== | ||
The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system. | The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system. | ||
Revision as of 15:59, 3 October 2013
No part name specified with partinfo tag.
construction
The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system.
biology
The plasmid pJD427 contains the two parts of splitted SspB, which can be fused (and thus regain function) by addition of rapamycin.
plac is a IPTG inducible promoter.
The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.
mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum at 610 nm.
The double terminator stops the transcription at this point.
application
This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ssrA-TT plasmid. [[1]]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1174
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 102
Illegal BsaI.rc site found at 743