Difference between revisions of "Part:BBa K1075028"

 
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<partinfo>BBa_K1075028 short</partinfo>
 
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===Usage and Biology===
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===construction===
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The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system.
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===biology===
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The plasmid pJD427 contains the two parts of splitted SspB, which can be fused (and thus regain function) by addition of rapamycin.
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plac is a IPTG inducible promoter.
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The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.
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mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum  at 610 nm.
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The double terminator stops the transcription at this point.
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===application===
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This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ssrA-TT plasmid. [[https://parts.igem.org/Part:BBa_K1075027]]
  
 
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Revision as of 15:56, 3 October 2013

SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-TT

a

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 102
    Illegal BsaI.rc site found at 743