Difference between revisions of "Part:BBa Y1000:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 9: Line 9:
 
Must eliminate biobrick sites + PvuII sites for use in transposome construction.
 
Must eliminate biobrick sites + PvuII sites for use in transposome construction.
  
−
CDE I concensus sequence: RTCACRG with the R's complementary to each other.
+
CDE I concensus sequence: RTCACRTG with the R's different
  
 
CDE II concensus sequence: AT rich > 90%  78-85 bp
 
CDE II concensus sequence: AT rich > 90%  78-85 bp

Latest revision as of 22:32, 3 August 2006


Yeast centromere sequence CEN4 from pYAC4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Must eliminate biobrick sites + PvuII sites for use in transposome construction.

CDE I concensus sequence: RTCACRTG with the R's different

CDE II concensus sequence: AT rich > 90% 78-85 bp

CDE III concensus sequence: WGTWYWTGWTTWCCGAANNWNWAAA

Source

pYAC4, Genbank U01086

References

Mann,C. and Davis,R.W. Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae Mol. Cell. Biol. 6 (1), 241-245 (1986) PMID: 3537685