Difference between revisions of "Part:BBa K1065101"

Revision as of 14:58, 3 October 2013

AraCpBAD + BSMT1

The part BBa_K1065100 is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when 5 mM of Arabinose is added to the culture.

This part was cloned by the iGEM UniTN Trento 2013 team for the construction of Wintergreen Devices BBa_K1065102 and BBa_K1065106.

Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 1893
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1434
Illegal SapI site found at 961

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	This part was cloned by the iGEM UniTN Trento 2013 team for the construction of		This part was cloned by the iGEM UniTN Trento 2013 team for the construction of
−	Wintergreen ~~Device~~ <partinfo>BBa_K1065106</partinfo>.	+	Wintergreen Devices <partinfo>BBa_K1065102</partinfo> and <partinfo>BBa_K1065106</partinfo>.