Difference between revisions of "Part:BBa K1033259"

Revision as of 14:54, 3 October 2013

Toxin-antitoxin system from lactobacillus plantarum

This is a toxin-antitoxin system originating from plasmid p256 from lactobacillus plantarum strain NC7. It has been created without the natural promotor to allow for customisable strength of the system. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been proven very efficient in previous studies (http://mic.sgmjournals.org/content/151/2/421.long).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 71
Illegal SpeI site found at 447
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 71
Illegal SpeI site found at 447
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 71
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 71
Illegal SpeI site found at 447
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 71
Illegal SpeI site found at 447
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1033259 short</partinfo>
-This is a toxin-antitoxin [system] originating from plasmid p256 from lactobacillus plantarum strain 256. (ref) It has been created without the natural promotor to allow for customisable strength of the system. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the  toxin will remain longer in the cell and thereby kill it. It has been proven very efficient in previous studies. (ref)
+This is a toxin-antitoxin system originating from plasmid p256 from lactobacillus plantarum strain NC7. It has been created without the natural promotor to allow for customisable strength of the system. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the  toxin will remain longer in the cell and thereby kill it. It has been proven very efficient in previous studies (http://mic.sgmjournals.org/content/151/2/421.long).
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